tRNA Fluctuations Observed on Stalled Ribosomes Are Suppressed during Ongoing Protein Synthesis
نویسندگان
چکیده
منابع مشابه
Protein synthesis factors (RF1, RF2, RF3, RRF, and tmRNA) and peptidyl-tRNA hydrolase rescue stalled ribosomes at sense codons.
During translation, ribosomes stall on mRNA when the aminoacyl-tRNA to be read is not readily available. The stalled ribosomes are deleterious to the cell and should be rescued to maintain its viability. To investigate the contribution of some of the cellular translation factors on ribosome rescuing, we provoked stalling at AGA codons in mutants that affected the factors and then analyzed the a...
متن کاملConformational changes in ribosomes during protein synthesis.
In a purified system containing poly(U) and ribosomal subunits from Escherichia coli, and purified transfer factors T and G, the active ribosomal complex passes through a cycle of contraction and expansion with the addition of each amino acid; aminoacyl-tRNA binding catalyzed by T produces the stable compact state, corresponding to the 70S conformation, whereas translocation with G expands the ...
متن کاملYaeJ is a novel ribosome-associated protein in Escherichia coli that can hydrolyze peptidyl–tRNA on stalled ribosomes
In bacteria, ribosomes often become stalled and are released by a trans-translation process mediated by transfer-messenger RNA (tmRNA). In the absence of tmRNA, however, there is evidence that stalled ribosomes are released from non-stop mRNAs. Here, we show a novel ribosome rescue system mediated by a small basic protein, YaeJ, from Escherichia coli, which is similar in sequence and structure ...
متن کاملTraffic of interacting ribosomes on mRNA during protein synthesis: effects of chemo-mechanics of individual ribosomes
Many ribosomes simultaneously move on the same messenger RNA (mRNA), each separately synthesizing the protein coded by the mRNA. Earlier models of ribosome traffic represent each ribosome by a “self-propelled particle” and capture the dynamics by an extension of the totally asymmetric simple exclusion process (TASEP). In contrast, here we develope a theoretical model that not only incorporates ...
متن کاملRibosomes and protein synthesis
Let us start at the very beginning. Between 1897 and 1899, G. Gamier, in France, published elegant microscope studies describing a basophilic component ofthe cytoplasm of glandular cells (1) . Because of what he thought its role might be in the elaboration and transformation of secretory products, he gave a Greek name to these concepts-ergastoplasm (work plasm) . Gamier's research was extended ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2017
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2017.08.052